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In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.cite-ref-1[1] Endcapped columns show decreased retention for hydrogen bond acceptors, such as ionized bases, and increased retention for protonated bases.cite-ref-2[2]

cite-note-11. ↑ citerefm-ndezboschros-sneue2003Méndez A, Bosch E, Rosés M, Neue UD (31 January 2003). "Comparison of the acidity of residual silanol groups in several liquid chromatography columns". Journal of Chromatography. 986 (1): 33–44. doi:10.1016/S0021-9673(02)01899-X. PMID 12585320.

cite-note-22. ↑ citerefwilsongilroydolansnyder2004Wilson NS, Gilroy J, Dolan JW, Snyder LR (13 February 2004). "Column selectivity in reversed-phase liquid chromatography: VI. Columns with embedded or end-capping polar groups". Journal of Chromatography. 1026 (1–2): 91–100. doi:10.1016/j.chroma.2003.11.041. PMID 14763736.